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Fuel wetting in the inlet port of a gasoline engine was studied using Laser-Induced Fluorescence (LIF). The measurements were done directly on the metal wall surface. Quantitative results were be obtained using a special calibration procedure. The sensitivity of the technique was found to correspond to a fuel layer thickness in the order of 1 μm, and the accuracy was estimated to be approx. 10 %. The engine was run on iso-octane, and in order to obtain fluorescence a dopant (3-pentanone) was added to the fuel. Laser light with a wave length of 266 nm was generated by frequency doubling the light from a Nd-YAG laser in two steps. A laser sheet was directed into the intake port and the fuel layer on the wall could be studied along a line on the bifurcation wall. The fluorescence light was detected with an intensified diode-array camera. The measurements from the fuel film thickness were compared with measurements of the total fuel film mass using an A/F response method.