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The combustion process in spark-ignition engines can vary considerably cycle by cycle, which may result in unstable engine operation. The phenomena amplify in natural gas (NG) spark-ignition (SI) engines due to the lower NG laminar flame speed compared to gasoline, and more so under lean burn conditions. The main goal of this study was to investigate the main sources and the characteristics of the cycle-by-cycle variation in heavy-duty compression ignition (CI) engines converted to NG SI operation. The experiments were conducted in a single-cylinder optically-accessible CI engine with a flat bowl-in piston that was converted to NG SI. The engine was operated at medium load under lean operating conditions, using pure methane as a natural gas surrogate. The CI to SI conversion was made through the addition of a low-pressure NG injector in the intake manifold and of a NG spark plug in place of the diesel injector.