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This article proposes a novel methodology for the definition of an optimized immersion cooling fluid for lithium-ion battery applications aimed to minimize maximum temperature and temperature gradient during most critical battery operations. The battery electric behavior is predicted by a first order equivalent circuit model, whose parameters are experimentally determined. Thermal behavior is described by a nodal network, assigning to each node thermal characteristics. Hence, the electro-thermal model of a battery is coupled with a thermal management model of an immersion cooling circuit developed in MATLAB/Simulink. A first characterization of the physical properties of an optimal dielectric liquid is obtained by means of a design of experiment. The optimal values of density, thermal conductivity, kinematic viscosity, and specific heat are defined to minimize the maximum temperature and temperature gradient during a complete discharge of the battery at 2.5C.