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The Single Cylinder Research Engine (SCRE) at the Institute of Internal Combustion Engines and Powertrain Systems is equipped with a variable valve train that allows to switch between regular intake valve lift and early intake valve closing (Miller). On the exhaust side, a secondary valve lift on each valve is possible with adjustable back pressure and thus the possibility of realising internal EGR. In combination with alternative fuels, even if they are Drop-In capable as HVO, properties differ and can influence the emission and efficiency behaviour. The investigations of this paper are focusing on regenerative Drop-In fuel (HVO), fossil fuel (B7), and an oxygenate (OME), that needs adaptions at the engine control unit, but offers further emission potential. By commissioning a 2-stage boost system, it is possible to fully equalize the air mass in Miller mode compared to the normal valve lift.