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The CANDS (Circulating, Aeration, and Nutrient Delivery System) Phase II SBIR is currently developing and testing methods and procedures to control moisture, oxygen, and temperature in the root zone of a particulate based micro-gravity nutrient delivery system. The completion of the first year and a half of the CANDS Phase II SBIR has shown significant engineering developments towards environmental control of the root zone. These developments include the measurement of root zone oxygen content, characterization of forced and flood-ebb aeration rates, successful control of root zone moisture using miniature heat-pulse moisture sensors, and successful control of root zone temperature via an insulating/temperature controlling water jacket. At the conclusion of the CANDS Phase II SBIR an integrated root zone environmental control system will be constructed for integration into plant growth systems to eliminate the uncertainties that exist in current plant growth data.

The Plant Research Unit (PRU) is the Space Station Biological Research Program plant growth facility being developed for the International Space Station. The plant habitat is designed for experiments in near-zero gravity or it can be rotated by the ISS Centrifuge for experiments at any gravity level from microgravity to twice Earth's gravity. Plant experimentation will be possible in multiple Plant Research Units at one time, isolating the effect of gravity on the biological specimens. The PRU will provide and control all aspects of a plant's needs in a nearly closed system. In other words, the shoot and root environments will not be open to the astronaut's environment except for experiment maintenance such as planting, harvesting and plant sampling. This also means that all lighting, temperature and humidity control, nutrient delivery, and air filtering and cleaning must be done in a very small volume, with very little mass and power usage and with minimal crew time.

The demand for highly flexible manipulation of plant growth generations, modification of specific plant processes, and genetically engineered crop varieties in a controlled environment has led to the development of a Commercial Plant Biotechnology Facility (CPBF). The CPBF is a quad-middeck locker playload to be mounted in the EXPRESS Rack that will be installed in the International Space Station (ISS). The CPBF integrates proven ASTROCULTURE” technologies, state-of-the-art control software, and fault tolerance and recovery technologies together to increase overall system efficiency, reliability, robustness, flexibility, and user friendliness. The CPBF provides a large plant growing volume for the support of commercial plant biotechnology studies and/or applications for long time plant research in a reduced gravity environment.

The ASTROCULTURE™ (ASC) middeck flight experiment series was developed to test subsystems required to grow plants in reduced gravity, with the goal of developing a plant growth unit suitable for conducting quality biological research in microgravity. Previous Space Shuttle flights (STS-50 and STS-57) have successfully demonstrated the ability to control water movement through a particulate rooting matrix in microgravity and the ability of LED lighting systems to provide high levels of irradiance without excessive heat build-up in microgravity. The humidity and temperature control system used in the middeck flight unit is described in this paper. The system controls air flow and provides dehumidification, humidification, and condensate recovery for a plant growth chamber volume of 1450 cm3.

Unique challenges arise during the design of temperature and humidity control systems (THCS) for use in microgravity. The design of the Plant Research Unit’s (PRU) THCS builds on the experience gained during the Biomass Production System (BPS) project and extends the understanding of the critical design variables and necessary technical advancements to allow for longer on-orbit operation. Previous systems have been limited by loss of prime, clogging in the porous plates and component reliability. Design of THCSs for long-duration space flight experiments requires the mitigation of these issues as well as a complete understanding of the relevant design variables. In addition to the normal design variables (e.g. mass, power, volume), a complex and interdependent relationship exists between the THCS variables including operational temperature range, operational humidity range, required humidity condensation rate and system air flow.

The advantages and disadvantages for the current oil consumption measurement systems, including the real-time oil consumption measurement and traditional weight methods, are reviewed. Based on the review, the Smart Oil Consumption Measuring System developed by Cummins Engine Co. in an effort to resolve some of the disadvantages of the systems developed earlier, especially compared to the Gravity Fed oil consumption measurement system, will be discussed. In addition, the uncertainty analysis of the Smart Oil Consumption Measuring System will also be briefly discussed here. The Smart Oil Consumption Measuring System has proven to be an effective tool to measure the oil consumption at almost any engine test conditions, including the steady and cyclic tests in a shorter time than most of traditional oil consumption measurement systems.

The ASTROCULTURE™ (ASC) middeck flight experiment series was developed to test and integrate subsystems required to grow plants in reduced gravity, with the goal of developing a plant growth unit suitable for conducting quality biological research in microgravity. Flights on the Space Shuttle have demonstrated control of water movement through a particulate rooting material, growth chamber temperature and humidity control, LED lighting systems and control, recycling of recovered condensate, ethylene scrubbing, and carbon dioxide control. A complete plant growth unit was tested on STS-63 in February 1995, the first ASC flight in which plant biology experiments were conducted in microgravity. The methods and objectives used for control of environmental conditions in the ASC unit are described in this paper.

The Biomass Production System, recently flown on the ISS for 73 days, demonstrated significant advancements in functional performance over previous systems for conducting plant science in microgravity. The Biomass Production System (BPS) was the first flight of a system with multiple, independently controlled, plant growth chambers. Each of four chambers was controlled separately with respect to temperature, humidity, light level, nutrient level, and CO2, and all were housed in a double Middeck locker-sized payload. During the mission, each of the subsystems performed within specification. This paper focuses on how the performance of the BPS hardware allowed successful completion of the preflight objectives.

The objective of the BPS Technology Validation Test (TVT) flown on the ISS as part of Increment 4 was to verify the functionality of environmental control subsystems and to measure the ability of the BPS to support plant growth and development in microgravity. Additional TVT objectives included validation of information acquisition systems, operations and support systems, and component performance. All TVT objectives were successfully addressed. Most evaluation criteria stipulated pre-flight were met. When there were deviations from pre-mission requirements, root causes were identified and subsystem configurations modified to eliminate these problems. Results from the TVT have been applied to the Plant Research Unit development to reduce technical risks and increase reliability. INTRODUCTION

The Advanced Animal Habitat (AAH) represents the next generation of Space Station based animal research facilities. Building upon previously developed flight hardware and experience, the AAH offers greatly enhanced system capabilities and performance. The design focuses upon the creation of a robust and flexible platform capable of supporting present and future experimental needs. A modular packaging and distributed control architecture leads to increased system adaptability and expandability. The baseline configuration includes group housing capability for up to six rats with automated food and water delivery as well as waste collection. Animals are continuously monitored with three cameras during both day and night cycles. The animals can be accessed while on-orbit through the Life Sciences Glovebox to perform a wide variety of experimental protocols.

A system was developed which provides nutrients and water to plants while maintaining good aeration at the roots and preventing water from escaping in reduced gravity. The nutrient solution is circulated through porous tubes under negative pressure and moves through the tube wall via capillary forces into the rooting matrix, establishing a non-saturated condition in the root zone. Tests using prototypes of the porous tube water and nutrient delivery system indicate that plant productivity in this system is equivalent to standard soil and solution culture growing procedures. The system has functioned successfully in short-term microgravity during parabolic flight tests and will be flown on the space shuttle. Plants are one of the components of a bioregenerative life support system required for long duration space missions.