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The development of virtual environment technology at NASA Ames Research Center and other research institutions has created opportunities for enhancing human performance. The application of this technology to training astronaut flight crews planning to go onboard the International Space Station has already begun at the NASA Johnson Space Center. A unique application of virtual environments to crew training is envisioned at NASA Ames Research Center which combines state of the art technology with haptic feedback to create a method for training crewmembers on critical life sciences operations which require fine motor skills. This paper describes such a concept, known as the Virtual Glovebox, as well as surveys other applications of virtual environments to astronaut crew training.

NASA is developing a Telescience Support Center (TSC) at the Ames Research Center. The center will be part of the infrastructure needed to conduct research in the Space Station and has been tailored to satisfy the requirements of the fundamental biology research program. The TSC will be developed from existing facilities at the Ames Research Center. Ground facility requirements have been derived from the TSC functional requirements. Most of the facility requirements will be satisfied with minor upgrades and modifications to existing buildings and laboratories. The major new development will be a modern data processing system. The TSC is being developed in three phases which correspond to deliveries of Biological Research Facility equipment to Station. The first phase will provide support for early hardware in flight Utilization Flight −1 (UF-1) in 2001.

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

The General Purpose Work Station (GPWS) is a laboratory multi-use facility, as demonstrated during the Spacelab Life Sciences 1 (SLS-1) flight. The unit provided particulate containment under varying conditions, served as an effective work space for manipulating live animals, e.g., rats, served as a containment facility for fixatives, and was proposed for use to conduct in-flight maintenance during connector pin repair. The cabinet has a front door large enough to allow installation of a full-size microscope in-flight and is outfitted with a side window to allow delivery of items into the cabinet without exposure to the spacelab atmosphere. Additional support subsystems include inside cabinet mounting, surgical glove fine manipulations capability, and alternating or direct current power supply for experiment equipment, as will be demonstrated during Spacelab J.

As part of its integrated system test bed capability, NASA's Advanced Life Support Program has undertaken the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. This facility--the Advanced Life Support Human-Rated Test Facility (HRTF) is currently being built at the Johnson Space Center. The HRTF is comprised of a series of interconnected chambers with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system will consist of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated command and control functions. Currently, a portion of this multichamber facility has been constructed and is being outfitted with basic utilities and infrastructure.

Ames Research Center's Life Sciences Division was responsible for managing the development of fundamental biology flight experiments during the Phase 1 NASA/Mir Science Program. Beginning with astronaut Norm Thagard's historic March, 1995 Soyuz rendezvous with the Mir station and continuing through Andy Thomas' successful return from Mir onboard STS-91 in June, 1998, the NASA/Mir Science Program has provided scientists with unparalleled long duration research opportunities. In addition, the Phase 1 program has yielded many valuable lessons to program and project management personnel who are managing the development of future International Space Station payload elements. This paper summarizes several of the key space station challenges faced and associated lessons learned by the Ames Research Center Fundamental Biology Research Project.

Determining the fundamental role of gravity in vital biological systems in space is one of six science and research areas that provides the philosophical underpinning for why NASA exists. The study of cells, tissues, and microorganisms in a spaceflight environment holds the promise of answering multiple intriguing questions about how gravity affects living systems. To enable these studies, specimens must be maintained in an environment similar to that used in a laboratory. Cell culture studies under normal laboratory conditions involve maintaining a highly specialized environment with the necessary temperature, humidity control, nutrient, and gas exchange conditions. These same cell life support conditions must be provided by the International Space Station (ISS) Cell Culture Unit (CCU) in the unique environment of space. The CCU is a perfusion-based system that must function in microgravity, at unit gravity (1g) on earth, and from 0.1g up to 2g aboard the ISS centrifuge rotor.

Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.

Poly (vinyl chloride) (PVC) matrix membranes which incorporate the ionophore nonactin have been evaluated as cation exchange membranes for ammonium ion transport in an electrolytic cell configuration. Interest exists for the development of cation selective membranes for removal of low levels (<200ppm) of ammonium ions commonly found in recycled effluent streams in such diverse applications as expected in a Space Station and commercial fisheries. Ammonium ions are generated as a decomposition product of urea and over time build up in concentration, thus rendering the water unsuitable for human consumption. Nonactin is commonly used in a PVC matrix for ion-selective electrodes.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

Biomolecules exhibit specific binding and high affinity for their ligands. These properties can be exploited to produce sensitive, specific, real-time sensors for analytes that cannot be readily monitored by other methods. Several technologies for environmental monitoring using proteins are currently being developed. We discuss specific challenges to practical application of a family of protein-based sensors derived from bacterial periplasmic binding proteins. We also present recent work to address these challenges.

NASA's Advanced Life Support Program has included as part of its long-range planning the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. As the designated NASA Field Center responsible for integration and testing of advanced life support systems, Johnson Space Center has undertaken the development of such a facility--the Advanced Life Support Human-Rated Test Facility (HRTF). As conceived, the HRTF is an interconnected five-chamber facility with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system which sustains the crew consists of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated control and monitoring functions.

Integrating physicochemical and biological technologies into a regenerative life support system is a complex technical challenge. NASA recognizes that the depth and breadth of the challenge warrants a comprehensive investigation. NASA is implementing several ground-based projects to look at different systems integration issues. The combined efforts of these activities will enable NASA to develop regenerative life support systems for human exploration of the solar system in the 21st century. This paper provides an overview of NASA's overall approach to ground testing of integrated regenerative life support systems.

Spacelab Life Sciences-3 (SLS-3) was scheduled to be the first United States man-tended microgravity flight containing Rhesus monkeys. The goal of this flight as in the five untended Russian COSMOS Bion flights and an earlier American Biosatellite flight, was to understand the biomedical and biological effects of a microgravity environment using the non-human primate as human surrogate. The SLS-3/Rhesus Project and COSMOS Primate-BIOS flights all utilized the rhesus monkey, Macaca mulatta. The ultimate objective of all flights with an animal surrogate has been to evaluate and understand biological mechanisms at both the system and cellular level, thus enabling rational effective countermeasures for future long duration human activity under microgravity conditions and enabling technical application to correction of common human physiological problems within earth's gravity, e.g., muscle strength and reloading, osteoporosis, immune deficiency diseases.

The Plant Generic BioProcessing Apparatus (PGBA), a plant growth facility developed for commercial space biotechnology research, has flown successfully on 3 spaceflight missions for 4, 10 and 16 days. The environmental control systems of this plant growth chamber (28 liter/0.075 m2) provide atmospheric, thermal, and humidity control, as well as lighting and nutrient supply. Typical performance profiles of water transpiration and dehumidification, carbon dioxide absorption (photosynthesis) and respiration rates in the PGBA unit (on orbit and ground) are presented. Data were collected on single and mixed crops. Design options and considerations for the different sub-systems are compared with those of similar hardware.

An integrated water recovery system was operated for 91 days in support of the Lunar Mars Life Support Test Project (LMLSTP) Phase III test. The system combined both biological and physical-chemical processes to treat a combined wastewater stream consisting of waste hygiene water, urine, and humidity condensate. Biological processes were used for primary degradation of organic material as well as for nitrification of ammonium in the wastewater. Physical-chemical systems removed inorganic salts from the water and provided post-treatment. The integrated system provided potable water to the crew throughout the test. This paper describes the water recovery system and reviews the performance of the system during the test.

The Centrifuge Accommodation Module (CAM) will be the home of the fundamental biology research facilities on the International Space Station (ISS). These facilities are being built by the Biological Research Project (BRP), whose goal is to oversee development of a wide variety of habitats and host systems to support life sciences research on the ISS. The habitats and host systems are designed to provide life support for a variety of specimens including cells, bacteria, yeast, plants, fish, rodents, eggs (e.g., quail), and insects. Each habitat contains specimen chambers that allow for easy manipulation of specimens and alteration of sample numbers. All habitats are capable of sustaining life support for 90 days and have automated as well as full telescience capabilities for sending habitat parameters data to investigator homesite laboratories.

Astrobiology is one of the most highly integrative scientific efforts ever undertaken, relying on the synthesis of sciences from astronomy to zoology and geology to genomics to discover the thread of life in the universe. These sciences must be further integrated with the technological revolutions in biotechnology, microminiaturization and information technology to realize the vast potential offered by NASA's mission suites. This paper discusses development of the Astrobiology Roadmap and novel management approaches which attempt to bring in the best scientific and technical talent available to bear on Astrobiology's goals, while simultaneously minimizing the overhead and time to flight for Astrobiology payloads.

This paper provides an overview of the IMMWPS Integrated Ground Test Program, completed at the NASA Johnson Space Center (JSC) during October and November 2001. The JSC Crew and Thermal Systems Division (CTSD) has developed the IMMWPS orbital flight experiment to test the feasibility of a microbe-based water purifier for use in zero-gravity conditions. The IMMWPS design utilizes a Microbial Processor Assembly (MPA) inoculated with facultative anaerobes to convert organic contaminants in wastewater to carbon dioxide and biomass. The primary purpose of the ground test program was to verify functional operations and procedures. A secondary objective was to provide initial ground data for later comparison to on-orbit performance. This paper provides a description of the overall test program, including the test article hardware and the test sequence performed to simulate the anticipated space flight test program. In addition, a summary of significant results from the testing is provided.

One of the key questions addressed in the field of Astrobiology is based upon the assumption that life exists, or at one time existed, in locations throughout the universe. However, this assumption is just that, an assumption. No definitive proof exists. On Earth, life has been found to exist in many diverse environments. We believe that this tendency towards diversity supports the assumption that life could exist wherever environmental conditions are right to support it. There are several locations within our Solar System which might support environments which are conducive to life. However, to conclusively establish the existence of life in such locations we must be capable of sensing generic life forms. This paper provides a summary of several innovative techniques based on the use of fluorescent analysis for the direct detection of extraterrestrial life forms.