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In recent times regulatory pressure to reduce CO2 emissions has driven research towards looking at blending fossil fuels with alternatives such as crop-produced alcohols. The alcohol of interest in this paper is ethanol and it was studied in mixtures with gasoline and iso-octane in an optical spark-ignition engine, running at 1500 RPM at low-load operation with 0.5 bar absolute intake plenum pressure. Specifically, tests involved fuels of 100% gasoline and 100% iso-octane, so that differences between multi and single-component fuels could be compared within this environment. A mixture of 25% ethanol with 75% iso-octane was also tested and compared. Finally, mixtures of high-percentage of ethanol (85% ethanol) in gasoline and in iso-octane were used in the study and compared. Tests were undertaken using a standard port injection system as well as a direct injection system so an appraisal of both mixture preparation methods could be made.